High Throughput Fingerprint Analysis of Large-Insert Clones
نویسندگان
چکیده
منابع مشابه
High throughput fingerprint analysis of large-insert clones.
As part of the Human Genome Project, the Washington University Genome Sequencing Center has commenced systematic sequencing of human chromsome 7. To organize and supply the effort, we have undertaken the construction of sequence-ready physical maps for defined chromosomal intervals. Map construction is a serial process composed of three main activities. First, candidate STS-positive large-inser...
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Physical mapping with large-insert clones is becoming an active area of genomics research, and capillary electrophoresis (CE) promises to revolutionize the physical mapping technology. Here, we demonstrate the utility of the CE technology for genome physical mapping with large-insert clones by constructing a robust, binary bacterial artificial chromosome (BIBAC)-based physical map of Penicilliu...
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A major challenge to positional cloning approaches is the identification of coding sequences within a region of interest. Hybridization of genomic fragments that represent a cloned contig of a defined genomic region in appropriate cDNA libraries theoretically represents a direct solution to this problem. However, this is technically difficult and in general, success with this approach has been ...
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We have designed and constructed a Fourier Transform Spectrometer (FTS) for the study of submillimeter-wave mixers and optical components. The FTS has a large aperture (up to 25.4 cm) and small focal ratio (as fast as f/2.5) to achieve a large throughput. It operates in the 100-3750 GHz (3.3-125 cm ?1) frequency range with a resolution of up to 75 MHz (0.0025 cm ?1). Here we discuss the design ...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1997
ISSN: 1088-9051,1549-5469
DOI: 10.1101/gr.7.11.1072